A new ultradian rhythm in mammalian cell dry mass observed by holography

Abstract We have discovered a new 4 h ultradian rhythm that occurs during the interphase of cell cycle in wide range individual mammalian cells, including both primary and transformed cells. The was detected by holographic lens-free microscopy follows histories dry mass thousands single live cells simultaneously, each at resolution five minutes. It vital observed inherently heterogeneous populations, thus eliminating synchronization labeling bias. is independent circadian rhythm, temperature-compensated. show amplitude fundamental frequency provides way to quantify effects of, chemical reagents on shedding light its mechanism. suppressed proteostasis disruptors only proliferating suggesting it represents massive degradation re-synthesis protein every growing